ISG15/UCRP Antibody (239)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90146
Recombinant Monoclonal Antibody
![Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146]](https://resources.bio-techne.com/images/products/ISG15-UCRP-Antibody-239-Western-Blot-NBP2-90146-img0002.jpg "Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 239
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human ISG15/UCRP (Accession#: AAH09507.1; Met1-Ser165).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ISG15/UCRP Antibody (239)
Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146]
Western Blot: ISG15/UCRP Antibody (239) [NBP2-90146] - Lane A: Hela Whole Cell LysateLane B: 293 Whole Cell LysateLane C: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:15 kDa![Immunocytochemistry/ Immunofluorescence: ISG15/UCRP Antibody (239) [NBP2-90146]](https://resources.bio-techne.com/images/products/ISG15-UCRP-Antibody-239-Immunocytochemistry-Immunofluorescence-NBP2-90146-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ISG15/UCRP Antibody (239) [NBP2-90146]")
Immunocytochemistry/ Immunofluorescence: ISG15/UCRP Antibody (239) [NBP2-90146]
Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (239) [NBP2-90146] - Immunofluorescence staining of Human ProISG15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ProISG15 monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluo(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.Applications for ISG15/UCRP Antibody (239)
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ISG15/UCRP
Long Name
ISG15 Ubiquitin-like Modifier
Alternate Names
G1P2, IFI15, IP17, UCRP
Gene Symbol
ISG15
Additional ISG15/UCRP Products
Product Documents for ISG15/UCRP Antibody (239)
Product Specific Notices for ISG15/UCRP Antibody (239)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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