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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E5

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant human ISG15/UCRP (1-157aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ISG15/UCRP Antibody (3E5) - BSA Free

Western Blot: ISG15/UCRP Antibody (3E5)BSA Free [NBP1-04310]

Western Blot: ISG15/UCRP Antibody (3E5)BSA Free [NBP1-04310]

Western Blot: ISG15/UCRP Antibody (3E5) [NBP1-04310] - Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate
Immunocytochemistry/ Immunofluorescence: ISG15/UCRP Antibody (3E5) - BSA Free [NBP1-04310]

Immunocytochemistry/ Immunofluorescence: ISG15/UCRP Antibody (3E5) - BSA Free [NBP1-04310]

Immunocytochemistry/Immunofluorescence: ISG15/UCRP Antibody (3E5) [NBP1-04310] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for ISG15/UCRP Antibody (3E5) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ISG15/UCRP

ISG15, a 15 kDa protein encoded by an interferon stimulated gene, and its modification system play important roles in the innate immune response, regulation of interferon signaling, pregnancy, and several cancers. ISG15 is an ubiquitin-like protein containing two ubiquitin homology domains and becomes conjugated to a variety of proteins when cells are treated with type I interferon or lipopolysaccharide.

Long Name

ISG15 Ubiquitin-like Modifier

Alternate Names

G1P2, IFI15, IP17, UCRP

Gene Symbol

ISG15

Additional ISG15/UCRP Products

Product Documents for ISG15/UCRP Antibody (3E5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ISG15/UCRP Antibody (3E5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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