Islet-1 Antibody (1A1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26193
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1A1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Islet-1 Antibody (1A1)
Immunogen
A synthesized peptide derived from Human Islet-1 [UniProt P61371]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Islet-1 Antibody (1A1)
Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] -
Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] - Image of Islet-1 Antibody (1A1) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] -
Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] - Image of Islet-1 Antibody (1A1) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: Islet-1 Antibody (1A1) [NBP3-26193] -
Western Blot: Islet-1 Antibody (1A1) [NBP3-26193] - Positive Western Blot detected in: 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate.All lanes: Islet-1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Applications for Islet-1 Antibody (1A1)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Islet-1
Alternate Names
ISL1, Islet1
Gene Symbol
ISL1
Additional Islet-1 Products
Product Documents for Islet-1 Antibody (1A1)
Product Specific Notices for Islet-1 Antibody (1A1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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