Isoleucyl tRNA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13037

Novus Biologicals, part of Bio-Techne
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NBP3-13037
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (94%), Mouse (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Isoleucyl tRNA synthetase Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Isoleucyl tRNA synthetase. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

144 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Isoleucyl tRNA synthetase Antibody

Western Blot: Isoleucyl tRNA synthetase Antibody [NBP3-13037]

Western Blot: Isoleucyl tRNA synthetase Antibody [NBP3-13037]

Western Blot: Isoleucyl tRNA synthetase Antibody [NBP3-13037] - Isoleucyl tRNA synthetase antibody detects Isoleucyl tRNA synthetase protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Isoleucyl tRNA synthetase antibody (NBP3-13037) diluted at 1:4000.
Immunohistochemistry-Paraffin: Isoleucyl tRNA synthetase Antibody [NBP3-13037]

Immunohistochemistry-Paraffin: Isoleucyl tRNA synthetase Antibody [NBP3-13037]

Immunohistochemistry-Paraffin: Isoleucyl tRNA synthetase Antibody [NBP3-13037] - Rat brain. Isoleucyl tRNA synthetase antibody (NBP3-13037) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: Isoleucyl tRNA synthetase Antibody [NBP3-13037]

Immunoprecipitation: Isoleucyl tRNA synthetase Antibody [NBP3-13037]

Immunoprecipitation: Isoleucyl tRNA synthetase Antibody [NBP3-13037] - Immunoprecipitation of Isoleucyl tRNA synthetase protein from 293T whole cell extracts using 5 ug of Isoleucyl tRNA synthetase antibody (NBP3-13037). Western blot analysis was performed using Isoleucyl tRNA synthetase antibody (NBP3-13037) diluted at 1:2500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Applications for Isoleucyl tRNA synthetase Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Isoleucyl tRNA synthetase

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq]

Alternate Names

EC 6.1.1, EC 6.1.1.5, FLJ20736, IARS1, ILERS, ILRS, IRS, isoleucine tRNA ligase 1, cytoplasmic, Isoleucine--tRNA ligase, isoleucyl-tRNA synthetase, isoleucyl-tRNA synthetase, cytoplasmic, PRO0785

Gene Symbol

IARS1

Additional Isoleucyl tRNA synthetase Products

Product Documents for Isoleucyl tRNA synthetase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Isoleucyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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