Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2E3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Isopeptidase T/USP5 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human USP5 (NP_003472) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

93.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free

Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3)Azide and BSA Free [NBP2-71355]

Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3)Azide and BSA Free [NBP2-71355]

Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5.
Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355]

Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355]

Immunohistochemistry: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Staining of paraffin-embedded Human pancreas tissue using anti-USP5 mouse monoclonal antibody.
Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3)Azide and BSA Free [NBP2-71355]

Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3)Azide and BSA Free [NBP2-71355]

Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free [NBP2-71355] - Analysis of extracts (35ug) from 9 different cell lines by using anti-USP5 monoclonal antibody.
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Applications for Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Isopeptidase T/USP5

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome. Alternate names for USP include ubiquitin carboxyl-terminal hydrolase 5, ubiquitin thioesterase 5, ubiquitin-specific-processing protease 5, deubuiquitinating enzyme 5, isopeptidase T, and ISOT.

Long Name

Ubiquitin Specific Protease 5

Alternate Names

IsoT, USP5

Gene Symbol

USP5

Additional Isopeptidase T/USP5 Products

Product Documents for Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Isopeptidase T/USP5 Antibody (OTI2E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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