IST1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33720

Novus Biologicals, part of Bio-Techne
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NBP2-33720
NBP2-33720-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IST1 Antibodies »

Product Summary for IST1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IST1 Antibody

Western Blot: IST1 Antibody [NBP2-33720]

Western Blot: IST1 Antibody [NBP2-33720]

Western Blot: IST1 Antibody [NBP2-33720] - Analysis using Anti-IST1 antibody NBP2-33720 (A) shows similar pattern to independent antibody NBP1-87676 (B).
Immunocytochemistry/ Immunofluorescence: IST1 Antibody [NBP2-33720]

Immunocytochemistry/ Immunofluorescence: IST1 Antibody [NBP2-33720]

Immunocytochemistry/Immunofluorescence: IST1 Antibody [NBP2-33720] - Immunofluorescent staining of human cell line MCF7 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: IST1 Antibody [NBP2-33720]

Immunohistochemistry-Paraffin: IST1 Antibody [NBP2-33720]

Immunohistochemistry-Paraffin: IST1 Antibody [NBP2-33720] - Staining of human liver shows no positivity in hepatocytes as expected.
View 3 more images

Applications for IST1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IST1

Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells.

Alternate Names

increased sodium tolerance 1 homolog (yeast), IST1 homolog, OLC1

Gene Symbol

IST1

UniProt

P53990 (Human)

Additional IST1 Products

Product Documents for IST1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IST1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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