ITCH/AIP4 Antibody (7I7B10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16669

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16669-100ul
NBP3-16669-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7I7B10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ITCH/AIP4 Antibodies »

Product Summary for ITCH/AIP4 Antibody (7I7B10)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ITCH/AIP4 (Q96J02). MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAAL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ITCH/AIP4 Antibody (7I7B10)

Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669]

Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669]

Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts of various cell lines, using ITCH/AIP4 Rabbit mAb (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669]

Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669]

Western Blot: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts of various cell lines, using ITCH/AIP4 Rabbit mAb (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Knockout Validated: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669]

Knockout Validated: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669]

Knockout Validated: ITCH/AIP4 Antibody (7I7B10) [NBP3-16669] - Analysis of extracts from normal (control) and ITCH/AIP4 knockout (KO) 293T cells, using ITCH/AIP4 antibody (NBP3-16669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for ITCH/AIP4 Antibody (7I7B10)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ITCH/AIP4

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Long Name

Itchy E3 Ubiquitin Protein Ligase

Alternate Names

AIF4, AIP4, NAPP1

Gene Symbol

ITCH

Additional ITCH/AIP4 Products

Product Documents for ITCH/AIP4 Antibody (7I7B10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ITCH/AIP4 Antibody (7I7B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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