ITCH/AIP4 Antibody (9C3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15151

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15151
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9C3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ITCH/AIP4 Antibodies »

Product Summary for ITCH/AIP4 Antibody (9C3)

Immunogen

A synthesized peptide derived from human ITCH/AIP4. UniProt ID (Q96J02).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ITCH/AIP4 Antibody (9C3)

Western Blot: ITCH/AIP4 Antibody (9C3) [NBP3-15151]

Western Blot: ITCH/AIP4 Antibody (9C3) [NBP3-15151]

Western Blot: ITCH/AIP4 Antibody (9C3) [NBP3-15151] - Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate, THP-1 whole cell lysate. All lanes: ITCH/AIP4 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 103, 99, 87 KDa. Observed band size: 103 KDa.

Applications for ITCH/AIP4 Antibody (9C3)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: ITCH/AIP4

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Long Name

Itchy E3 Ubiquitin Protein Ligase

Alternate Names

AIF4, AIP4, NAPP1

Gene Symbol

ITCH

Additional ITCH/AIP4 Products

Product Documents for ITCH/AIP4 Antibody (9C3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ITCH/AIP4 Antibody (9C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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