ITK Antibody (HL1264) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25538
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (94%), Rat (93%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1264
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human ITK. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ITK Antibody (HL1264) - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] -
Immunocytochemistry/Immunofluorescence: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - ITK antibody [HL1264] detects ITK protein by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK stained by ITK antibody [HL1264] (NBP3-25538) diluted at 1:500. Blue: Fluoroshield with DAPI .
Western Blot: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] -
Western Blot: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [HL1264] (NBP3-25538) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] -
Immunohistochemistry-Paraffin: ITK Antibody (HL1264) - Azide and BSA Free [NBP3-25538] - ITK antibody [HL1264] detects ITK protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. ITK stained by ITK antibody [HL1264] (NBP3-25538) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for ITK Antibody (HL1264) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ITK
Long Name
IL-2-inducible T-cell Kinase
Alternate Names
EMT, LYK, PSCTK2, Tcsk
Gene Symbol
ITK
Additional ITK Products
Product Documents for ITK Antibody (HL1264) - Azide and BSA Free
Product Specific Notices for ITK Antibody (HL1264) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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