JAB1 Antibody (2A10.8)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-495

Novus Biologicals, part of Bio-Techne
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NB120-495
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2A10.8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all JAB1 Antibodies »

Product Specifications

Immunogen

The full-length mouse JAB-1 gene expressed in E. coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasmic and Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JAB1 Antibody (2A10.8)

Western Blot: JAB1 Antibody (2A10.8) [NB120-495]

Western Blot: JAB1 Antibody (2A10.8) [NB120-495]

Western Blot: JAB1 Antibody (2A10) [NB120-495] - Sample (50 ug of whole cell lysate) A: Rat liver 10% SDS PAGE diluted at 1:1000
Immunohistochemistry: JAB1 Antibody (2A10) [NB120-495]
Western Blot: JAB1 Antibody (2A10.8) [NB120-495]

Western Blot: JAB1 Antibody (2A10.8) [NB120-495]

Western Blot: JAB1 Antibody (2A10) [NB120-495] - Jab1 antibody [2A10.8] Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE JAB1 Antibody diluted at 1:10000
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Applications for JAB1 Antibody (2A10.8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

0.5-1 ug/ml

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JAB1

Jun activation domain-binding protein 1 (JAB1) also designated COP9 subunit 5 (COPS5) or SGN5 is a coactivator of AP1 transcription factor that also promotes degradation of the cyclin-dependent kinase inhibitor p27Kip1. JAB1 interacts with c-Jun AP1 containing complexes, and enhances transactivation from AP1 dependent promoters. It also interacts with Jun D but not with Jun B or v-Jun. JAB1 is highly conserved in evolution and is widely expressed in mammalian tissues. It is localized both the nucleus and the cytoplasm. It interacts with the cytoplasmic domain of the alphaL/beta2 integrin LFA1. Following LFA1 engagement the nuclear pool of JAB1 increases and activation of an AP1 driven promoter is enhanced. Interaction of JAB1 with the nuclear progesterone receptor and the steroid receptor activator (SRC1) was reported. JAB1 is a stability and activity regulator of Hypoxia inducible factor 1 (HIF1), a transcription factor that controls activation of several genes responsive to the cellular oxygen tension. The macrophage migration inhibitory factor (MIF) associates with JAB1 in the cytosol near the plasma membrane. Endogenous MIF inhibits JAB1 induced AP1 transcriptional activity. JAB1 is a subunit of the COP9 regulatory complex. COP9 cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. A metalloprotease motif in JAB1 plays a role in this isopeptidase activity. Breakdown of the cyclin dependent kinase inhibitor p27Kip1 is promoted by JAB1. The latter expression in several cancers inversely correlates with p27Kip1 and may reflect tumor aggressiveness. A possible involvement of JAB1 in atherosclerosis was also reported. Involvement of JAB1 in degradation of the suppressors p53 and smad4 was described recently.

Long Name

Jun Activation Domain-binding Protein 1

Alternate Names

COPS5, CSN5, MOV-34, SGN5

Entrez Gene IDs

10987 (Human); 26754 (Mouse); 117039 (Rat)

Gene Symbol

COPS5

UniProt

Q92905 (Human)

Additional JAB1 Products

Product Documents for JAB1 Antibody (2A10.8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JAB1 Antibody (2A10.8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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