JAB1 Antibody (3X5F0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16328
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation (ChIP), ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3X5F0
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human JAB1 (Q92905). MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for JAB1 Antibody (3X5F0)
Western Blot: JAB1 Antibody (3X5F0) [NBP3-16328]
Western Blot: JAB1 Antibody (3X5F0) [NBP3-16328] - Western blot analysis of extracts of various cell lines, using JAB1 Rabbit mAb (NBP3-16328) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunocytochemistry/ Immunofluorescence: JAB1 Antibody (3X5F0) [NBP3-16328]
Immunocytochemistry/Immunofluorescence: JAB1 Antibody (3X5F0) [NBP3-16328] - Immunofluorescence analysis of U-2 OS cells using JAB1 Rabbit mAb (NBP3-16328) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: JAB1 Antibody (3X5F0) [NBP3-16328]
Immunohistochemistry-Paraffin: JAB1 Antibody (3X5F0) [NBP3-16328] - Immunohistochemistry of paraffin-embedded mouse testis using JAB1 Rabbit mAb (NBP3-16328) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for JAB1 Antibody (3X5F0)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
5μg antibody for 10μg-15μg of Chromatin
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: JAB1
Long Name
Jun Activation Domain-binding Protein 1
Alternate Names
COPS5, CSN5, MOV-34, SGN5
Gene Symbol
COPS5
Additional JAB1 Products
Product Documents for JAB1 Antibody (3X5F0)
Product Specific Notices for JAB1 Antibody (3X5F0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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