JAB1 Antibody (OTI2B12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01813

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B12

Concentration

0.76 mg/ml

View all JAB1 Antibodies »

Product Summary for JAB1 Antibody (OTI2B12)

Immunogen

Full length human recombinant protein of human COPS5(NP_006828) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JAB1 Antibody (OTI2B12)

Western Blot: JAB1 Antibody (OTI2B12) [NBP2-01813]

Western Blot: JAB1 Antibody (OTI2B12) [NBP2-01813]

Western Blot: JAB1 Antibody (2B12) [NBP2-01813] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY COPS5 (RC200979, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiCOPS
Immunocytochemistry/ Immunofluorescence: JAB1 Antibody (OTI2B12) [NBP2-01813]

Immunocytochemistry/ Immunofluorescence: JAB1 Antibody (OTI2B12) [NBP2-01813]

Immunocytochemistry/Immunofluorescence: JAB1 Antibody (2B12) [NBP2-01813] - Anti-COPS5 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COPS5(RC200979).
Immunohistochemistry-Paraffin: JAB1 Antibody (OTI2B12) [NBP2-01813]

Immunohistochemistry-Paraffin: JAB1 Antibody (OTI2B12) [NBP2-01813]

Immunohistochemistry-Paraffin: JAB1 Antibody (OTI2B12) [NBP2-01813] - Staining of paraffin-embedded Carcinoma of Human kidney tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.
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Applications for JAB1 Antibody (OTI2B12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.76 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JAB1

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq].

Long Name

Jun Activation Domain-binding Protein 1

Alternate Names

COPS5, CSN5, MOV-34, SGN5

Entrez Gene IDs

10987 (Human)

Gene Symbol

COPS5

UniProt

Q92905 (Human)

Additional JAB1 Products

Product Documents for JAB1 Antibody (OTI2B12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for JAB1 Antibody (OTI2B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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