Jak1 [p Tyr1022] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82005

Novus Biologicals, part of Bio-Techne
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NB100-82005
NB100-82005-0.025ml
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Jak1 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human JAK1 around the phosphorylation site of tyrosine 1022 (K-E-YP-Y-T).

Modification

p Tyr1022

Specificity

Detects endogenous levels of Jak1 only when phosphorylated at tyrosine 1022.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

132 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Jak1 [p Tyr1022] Antibody

Western Blot: Jak1 [p Tyr1022] Antibody [NB100-82005] - Analysis of extracts from HT29 cells untreated or treated with UV using JAK1 [p Tyr1022] antibody.

Immunohistochemistry-Paraffin: Jak1 [p Tyr1022] Antibody [NB100-82005] -

Immunohistochemistry-Paraffin: Jak1 [p Tyr1022] Antibody [NB100-82005] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82005 (left) or the same antibody preincubated with blocking peptide (right).

Applications for Jak1 [p Tyr1022] Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:300

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:200-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% glycerol, 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Jak1

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterised by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signalling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.

Long Name

Janus Kinase 1

Alternate Names

EC 2.7.10, JAK-1, JAK1Atyrosine-protein kinase JAK1, JAK1B, Janus kinase 1EC 2.7.10.2, JTK3

Entrez Gene IDs

3716 (Human); 16451 (Mouse); 84598 (Rat)

Gene Symbol

JAK1

UniProt

P23458

Additional Jak1 Products

Product Documents for Jak1 [p Tyr1022] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Jak1 [p Tyr1022] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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