Jak1 [p Tyr1022, p Tyr1023] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05436

Novus Biologicals, part of Bio-Techne
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NBP3-05436
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Jak1 Antibodies »

Product Summary for Jak1 [p Tyr1022, p Tyr1023] Antibody - BSA Free

Immunogen

A synthetic phosphorylated peptide around Y1022 & Y1023 of human JAK1 (NP_002218.2). KEYYTV

Modification

p Tyr1022, p Tyr1023

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Jak1 [p Tyr1022, p Tyr1023] Antibody - BSA Free

Western Blot: Jak1 [p Tyr1022, p Tyr1023] Antibody [NBP3-05436] - Western blot analysis of extracts of RAW264.7 cells, using Jak1 antibody (NBP3-05436) at 1:1000 dilution.RAW264.7 cells were treated by Pervanadate (1 mM) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: Jak1 [p Tyr1022, p Tyr1023] Antibody - BSA Free [NBP3-05436]

Immunocytochemistry/ Immunofluorescence: Jak1 [p Tyr1022, p Tyr1023] Antibody - BSA Free [NBP3-05436]

Immunocytochemistry/Immunofluorescence: Jak1 [p Tyr1022, p Tyr1023] Antibody [NBP3-05436] - Immunofluorescence analysis of NIH/3T3 cells using Jak1 antibody (NBP3-05436) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for Jak1 [p Tyr1022, p Tyr1023] Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Jak1

Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterised by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signalling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.

Long Name

Janus Kinase 1

Alternate Names

EC 2.7.10, JAK-1, JAK1Atyrosine-protein kinase JAK1, JAK1B, Janus kinase 1EC 2.7.10.2, JTK3

Gene Symbol

JAK1

Additional Jak1 Products

Product Documents for Jak1 [p Tyr1022, p Tyr1023] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Jak1 [p Tyr1022, p Tyr1023] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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