Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26415
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1A4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human JAK2 [p Tyr1007, p Tyr1008] [UniProt O60674]
Modification
p Tyr 1007, p Tyr 1008
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4)
Immunoprecipitation: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] -
Immunoprecipitation: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] - Immunoprecipitating Jak2 [p Tyr1007, p Tyr1008] in Hela whole cell lysate treated with Pervanadate.Lane 1: Rabbit control IgG (1ug) instead of NBP3-26415 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26415 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).
Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).
Immunohistochemistry: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] -
Immunohistochemistry: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] - Image of Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.Immunohistochemistry: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] -
Immunohistochemistry: Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) [NBP3-26415] - Image of Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.Applications for Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Jak2
Long Name
Janus Kinase 2
Alternate Names
EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2
Gene Symbol
JAK2
Additional Jak2 Products
Product Documents for Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4)
Product Specific Notices for Jak2 [p Tyr1007, p Tyr1008] Antibody (1A4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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