Jak2 Antibody (4B0K1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15825

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15825-100ul
NBP3-15825-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4B0K1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Jak2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1033-1132 of human Jak2 (O60674). VASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Jak2 Antibody (4B0K1)

Western Blot: Jak2 Antibody (4B0K1) [NBP3-15825]

Western Blot: Jak2 Antibody (4B0K1) [NBP3-15825]

Western Blot: Jak2 Antibody (4B0K1) [NBP3-15825] - Western blot analysis of extracts of various cell lines, using Jak2 antibody (NBP3-15825) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunoprecipitation: Jak2 Antibody (4B0K1) [NBP3-15825]

Immunoprecipitation: Jak2 Antibody (4B0K1) [NBP3-15825]

Immunoprecipitation: Jak2 Antibody (4B0K1) [NBP3-15825] - Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug Jak2 antibody (NBP3-15825). Western blot was performed from the immunoprecipitate using Jak2 antibody (NBP3-15825) at a dilition of 1:1000.

Applications for Jak2 Antibody (4B0K1)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Jak2

Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).

Long Name

Janus Kinase 2

Alternate Names

EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2

Gene Symbol

JAK2

Additional Jak2 Products

Product Documents for Jak2 Antibody (4B0K1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Jak2 Antibody (4B0K1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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