Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI7D12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Jak2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 297-596 of human JAK2(NP_004963) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Jak2 Antibody (OTI7D12) - Azide and BSA Free

Immunohistochemistry: Jak2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-71056]

Immunohistochemistry: Jak2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-71056]

Immunohistochemistry: Jak2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-71056] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: Jak2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-71056]

Immunohistochemistry: Jak2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-71056]

Immunohistochemistry: Jak2 Antibody (OTI7D12) - Azide and BSA Free [NBP2-71056] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min)

Applications for Jak2 Antibody (OTI7D12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Jak2

Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).

Long Name

Janus Kinase 2

Alternate Names

EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2

Gene Symbol

JAK2

Additional Jak2 Products

Product Documents for Jak2 Antibody (OTI7D12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Jak2 Antibody (OTI7D12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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