Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Jak2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1082 and 1132 of human Janus kinase 2 using the numbering given in entry NP_004963.1 (GeneID 3717).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Jak2 Antibody

Western Blot: Jak2 Antibody [NBP1-22975]

Western Blot: Jak2 Antibody [NBP1-22975]

Western Blot: Jak2 Antibody [NBP1-22975] - Whole cell lysate from Jurkat (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-JAK2 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. JAK2 was also immunoprecipitated by rabbit anti-JAK2 antibody BL3496, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunohistochemistry-Paraffin: Jak2 Antibody [NBP1-22975]

Immunohistochemistry-Paraffin: Jak2 Antibody [NBP1-22975]

Immunohistochemistry-Paraffin: Jak2 Antibody [NBP1-22975] - Human prostate carcinoma. Antibody: Affinity purified rabbit anti-JAK2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.

Applications for Jak2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 to 1:2000

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Jak2

JAK2 (Janus kinase 2) was originally cloned via screening of mouse cDNA libraries with PCR generated probes representing highly conserved elements in the protein tyrosine kinase (PTK) family. JAK2, as well as JAK, was named after the Roman god, Janus, the god of gates or doors, and who is depicted as having two faces which face opposite directions. JAK kinases bear two nearly identical phosphate transferring domains and function to transduce signals from type I and type II cytokine receptors for the activation of STAT (signal transducers and activators of transcription) transcription factors.

Long Name

Janus Kinase 2

Alternate Names

EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2

Entrez Gene IDs

3717 (Human)

Gene Symbol

JAK2

UniProt

O60674 (Human)

Additional Jak2 Products

Product Documents for Jak2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Jak2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.