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JAM-A Antibody (2E3-1C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00050848-M01

Novus Biologicals, part of Bio-Techne
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H00050848-M01

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E3-1C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

F11R (AAH01533, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGTKAQVERKLLCLFILAILLCSLALGSVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVIVAAVLVTLILLGILVFGIWFAYSRGHFDRTKKGTSSKKVIYSQPSARSEGEFKQTSSFLV

Reactivity Notes

Human. Other species not tested.

Localization

Type I membrane protein

Specificity

F11R - F11 receptor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for JAM-A Antibody (2E3-1C8)

Immunohistochemistry: JAM-A Antibody (2E3-1C8) [H00050848-M01]

Immunohistochemistry: JAM-A Antibody (2E3-1C8) [H00050848-M01]

Immunohistochemistry: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Coronal sections of human meninges including the retrobulbar, midorbital, and intracanalicular part of the optic nerve stained for cell-cell interaction markers of tight junctions (JAM-A, Occludin, and Claudin 5). A representative staining of one patient is shown. * indicates arachnoid layer. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fneur.2017.00308/full) licensed under a CC-BY license.
Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01]

Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01]

Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody (M01), clone 2E3-1C8. Lane 1: F11R transfected lysate (Predicted MW: 32.6 KDa). Lane 2: Non-transfected lysate.
Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01]

Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01]

Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - F11R monoclonal antibody (M01), clone 2E3-1C8 Analysis of F11R expression in HepG2.

Applications for JAM-A Antibody (2E3-1C8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: JAM-A

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Long Name

Junctional Adhesion Molecule A

Alternate Names

CD321, F11R, JAMA, PAM-1

Entrez Gene IDs

50848 (Human)

Gene Symbol

F11R

OMIM

605721 (Human)

Additional JAM-A Products

Product Documents for JAM-A Antibody (2E3-1C8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for JAM-A Antibody (2E3-1C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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