JAM-A Antibody (2E3-1C8)
Novus Biologicals, part of Bio-Techne | Catalog # H00050848-M01
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Sandwich ELISA, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E3-1C8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
F11R (AAH01533, 1 a.a. ~ 299 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGTKAQVERKLLCLFILAILLCSLALGSVTVHSSEPEVRIPENNPVKLSCAYSGFSSPRVEWKFDQGDTTRLVCYNNKITASYEDRVTFLPTGITFKSVTREDTGTYTCMVSEEGGNSYGEVKVKLIVLVPPSKPTVNIPSSATIGNRAVLTCSEQDGSPPSEYTWFKDGIVMPTNPKSTRAFSNSSYVLNPTTGELVFDPLSASDTGEYSCEARNGYGTPMTSNAVRMEAVERNVGVIVAAVLVTLILLGILVFGIWFAYSRGHFDRTKKGTSSKKVIYSQPSARSEGEFKQTSSFLV
Reactivity Notes
Human. Other species not tested.
Localization
Type I membrane protein
Specificity
F11R - F11 receptor
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for JAM-A Antibody (2E3-1C8)
Immunohistochemistry: JAM-A Antibody (2E3-1C8) [H00050848-M01]
Immunohistochemistry: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Coronal sections of human meninges including the retrobulbar, midorbital, and intracanalicular part of the optic nerve stained for cell-cell interaction markers of tight junctions (JAM-A, Occludin, and Claudin 5). A representative staining of one patient is shown. * indicates arachnoid layer. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fneur.2017.00308/full) licensed under a CC-BY license.Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01]
Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - Analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody (M01), clone 2E3-1C8. Lane 1: F11R transfected lysate (Predicted MW: 32.6 KDa). Lane 2: Non-transfected lysate.Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01]
Western Blot: JAM-A Antibody (2E3-1C8) [H00050848-M01] - F11R monoclonal antibody (M01), clone 2E3-1C8 Analysis of F11R expression in HepG2.Applications for JAM-A Antibody (2E3-1C8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: JAM-A
Long Name
Junctional Adhesion Molecule A
Alternate Names
CD321, F11R, JAMA, PAM-1
Entrez Gene IDs
50848 (Human)
Gene Symbol
F11R
OMIM
605721 (Human)
UniProt
Additional JAM-A Products
Product Documents for JAM-A Antibody (2E3-1C8)
Product Specific Notices for JAM-A Antibody (2E3-1C8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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