JMJD2B Antibody (8I0I1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16639

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16639-100ul
NBP3-16639-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8I0I1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all JMJD2B Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human JMJD2B (O94953). PPKEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRRLANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JMJD2B Antibody (8I0I1)

Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639]

Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639]

Western Blot: JMJD2B Antibody (8I0I1) [NBP3-16639] - Western blot analysis of extracts of various cell lines, using JMJD2B Rabbit mAb (NBP3-16639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunocytochemistry/Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of U-2 OS cells using JMJD2B Rabbit mAb (NBP3-16639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunocytochemistry/ Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639]

Immunocytochemistry/Immunofluorescence: JMJD2B Antibody (8I0I1) [NBP3-16639] - Immunofluorescence analysis of C6 cells using JMJD2B Rabbit mAb (NBP3-16639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for JMJD2B Antibody (8I0I1)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JMJD2B

JMJD2B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JMJD2B has been shown to be capable of antagonizing H3K9 trimethylation.

Long Name

Lysine-specific demethylase 4B

Alternate Names

EC 1.14.11.66, JHDM3B, KDM4B, KIAA0876

Gene Symbol

KDM4B

Additional JMJD2B Products

Product Documents for JMJD2B Antibody (8I0I1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JMJD2B Antibody (8I0I1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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