JNK1/2/3 Antibody (0J5D8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16250

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16250-100ul
NBP3-16250-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0J5D8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all JNK Antibodies »

Product Summary for JNK1/2/3 Antibody (0J5D8)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-383 of human JNK1 (P45983). MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLGAAV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JNK1/2/3 Antibody (0J5D8)

Western Blot: JNK1/2/3 Antibody (0J5D8) [NBP3-16250]

Western Blot: JNK1/2/3 Antibody (0J5D8) [NBP3-16250]

Western Blot: JNK1/2/3 Antibody (0J5D8) [NBP3-16250] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 Rabbit mAb (NBP3-16250) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry: JNK1/2/3 Antibody (0J5D8) [NBP3-16250]

Immunohistochemistry: JNK1/2/3 Antibody (0J5D8) [NBP3-16250]

Immunohistochemistry: JNK1/2/3 Antibody (0J5D8) [NBP3-16250] - Immunofluorescence analysis of mouse brain using JNK1/2/3 Rabbit mAb (NBP3-16250) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for JNK1/2/3 Antibody (0J5D8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JNK

JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun Nterminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.

Long Name

C-Jun N-terminal Kinase

Alternate Names

c-Jun N-terminal kinase 1, EC 2.7.11, EC 2.7.11.24, JNK1 alpha protein kinase, JNK1 beta protein kinase, JNK1JNK1A2, JNK-46, JUN N-terminal kinase, MAP kinase 8, MAPK 8, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, PRKM8JNK, protein kinase JNK1, SAPK1JNK21B1/2, Stress-activated protein kinase 1, Stress-activated protein kinase JNK1

Gene Symbol

MAPK8

Additional JNK Products

Product Documents for JNK1/2/3 Antibody (0J5D8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for JNK1/2/3 Antibody (0J5D8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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