JNK1 Antibody (8Q7S4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16536

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16536-100ul
NBP3-16536-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8Q7S4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all JNK1 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-383 of human JNK1 (P45983). MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLGAAV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JNK1 Antibody (8Q7S4)

Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536]

Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536]

Western Blot: JNK1 Antibody (8Q7S4) [NBP3-16536] - Western blot analysis of extracts of various cell lines, using JNK1/JNK3 Rabbit mAb (NBP3-16536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536]

Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536]

Immunocytochemistry/Immunofluorescence: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunofluorescence analysis of U-2 OS cells using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: JNK1 Antibody (8Q7S4) [NBP3-16536]

Immunohistochemistry-Paraffin: JNK1 Antibody (8Q7S4) [NBP3-16536]

Immunohistochemistry-Paraffin: JNK1 Antibody (8Q7S4) [NBP3-16536] - Immunohistochemistry of paraffin-embedded human colon using JNK1/JNK3 Rabbit mAb (NBP3-16536) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for JNK1 Antibody (8Q7S4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JNK1

JNK1, also known as MAPK1, is a JNK protein subgroup familymenber of the mitogen-activated protein kinase (MAPK) superfamily and is part of a signal transduction cascade that is activated by cytokines and by exposure to environmental stresses. Components of the JNK (c-Jun N-terminal kinase) signaling pathway have been shown to interact with the insulin signaling pathway and play a pivotal role in lifespan regulation and stress resistance in many diverse organisms. Therefore, JNK1 plays a role in regulating both life span and stress resistance.

Long Name

C-Jun N-terminal Kinase 1

Alternate Names

MAPK8, PRKM8, SAPK1

Gene Symbol

MAPK8

Additional JNK1 Products

Product Documents for JNK1 Antibody (8Q7S4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JNK1 Antibody (8Q7S4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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