Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4E3

Concentration

0.66 mg/ml

View all JNK1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-384 of human JNK1 (NP_002741) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JNK1 Antibody (OTI4E3)

Western Blot: JNK1 Antibody (OTI4E3) [NBP1-48284]

Western Blot: JNK1 Antibody (OTI4E3) [NBP1-48284]

Western Blot: JNK1 Antibody (4E3) [NBP1-48284] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-JNK1.
Immunocytochemistry/Immunofluorescence: JNK1 Antibody (4E3) [NBP1-48284] Staining of COS7 cells transiently transfected by pCMV6-ENTRY JNK1.
Immunohistochemistry-Paraffin: JNK1 Antibody (OTI4E3) [NBP1-48284]

Immunohistochemistry-Paraffin: JNK1 Antibody (OTI4E3) [NBP1-48284]

Immunohistochemistry-Paraffin: JNK1 Antibody (4E3) [NBP1-48284] - Staining of paraffin-embedded Human pancreas tissue using anti-JNK1 mouse monoclonal antibody.
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Applications for JNK1 Antibody (OTI4E3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.66 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JNK1

JNK1 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK1 is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of JNK1 by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. JNK1 is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of JNK1 gene suggested that it play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported

Long Name

C-Jun N-terminal Kinase 1

Alternate Names

MAPK8, PRKM8, SAPK1

Entrez Gene IDs

5599 (Human)

Gene Symbol

MAPK8

Additional JNK1 Products

Product Documents for JNK1 Antibody (OTI4E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for JNK1 Antibody (OTI4E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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