JNK2 Antibody (OTI1A1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71259

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all JNK2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MAPK9(NP_620707) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JNK2 Antibody (OTI1A1) - Azide and BSA Free

Western Blot: JNK2 Antibody (OTI1A1)Azide and BSA Free [NBP2-71259]

Western Blot: JNK2 Antibody (OTI1A1)Azide and BSA Free [NBP2-71259]

Western Blot: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY JNK2.
Immunocytochemistry/ Immunofluorescence: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259]

Immunocytochemistry/ Immunofluorescence: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259]

Immunocytochemistry/Immunofluorescence: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY JNK2.
Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259]

Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259]

Immunohistochemistry: JNK2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-71259] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for JNK2 Antibody (OTI1A1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JNK2

JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).

Long Name

C-Jun N-terminal Kinase 2

Alternate Names

MAPK9, SAPK1 alpha

Gene Symbol

MAPK9

Additional JNK2 Products

Product Documents for JNK2 Antibody (OTI1A1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for JNK2 Antibody (OTI1A1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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