JNK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99547

Novus Biologicals, part of Bio-Techne
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NBP2-99547-100ul
NBP2-99547-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all JNK2 Antibodies »

Product Summary for JNK2 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human JNK2 (Accession#: NP_002743.3; Met1-Arg424)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for JNK2 Antibody

Immunoprecipitation: JNK2 Antibody [NBP2-99547]

Immunoprecipitation: JNK2 Antibody [NBP2-99547]

Immunoprecipitation: JNK2 Antibody [NBP2-99547] - JNK2 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. Lane B: 0.5 mg MCF-7 Whole Cell Lysate. 2 ul anti-JNK2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-JNK2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa

Applications for JNK2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JNK2

JNK protein kinases are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group includes the 46-kDa isoform JNK1 and the 55-kDa protein kinase JNK2. The activities of both JNK isoforms are markedly increased by exposure of cells to UV radiation (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). JNK2 functions in a cell-type-specific and stimulus-dependent manner, being required for apoptosis of immature thymocytes induced by anti-CD3 antibody but not for apoptosis induced by anti-Fas antibody, UVC or dexamethasone. JNK2 is not required for activation-induced cell death of mature T cells (3).

Long Name

C-Jun N-terminal Kinase 2

Alternate Names

MAPK9, SAPK1 alpha

Gene Symbol

MAPK9

Additional JNK2 Products

Product Documents for JNK2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for JNK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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