Jumonji/JARID2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2214

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rabbit

Cited:

Human, Mouse, Rabbit

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Simple Western, Western Blot

Cited:

Chemotaxis, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Jumonji/JARID2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human JARID2 protein sequence (between residues 1-100). [UniProt# Q92833]

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24074864)

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Jumonji/JARID2 Antibody

Knockout Validated: Jumonji/JARID2 Antibody [NB100-2214]

Knockout Validated: Jumonji/JARID2 Antibody [NB100-2214]

Knockout Validated: Jumonji/JARID2 Antibody [NB100-2214] - Jumonji/JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes. Immunoblot of nucleosomal pull downs from nuclear extract. Total nuclear extract (input) and pull down using streptavidin beads, unmodified nucleosomes or nucleosomes modified by H2AK119u1/H2AK119u1(I44A) were probed with the indicated antibodies using extracts from WT cells (left) or Jumonji/JARID2 KO mESCs (right). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms13661) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody [NB100-2214]

Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody [NB100-2214]

Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: Jumonji/JARID2 Antibody [NB100-2214]

Western Blot: Jumonji/JARID2 Antibody [NB100-2214]

Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jarid2 expression in naive and activated (overnight) murine B cells. This image was submitted via customer Review.
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Applications for Jumonji/JARID2 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation

1:10-1:500. Use reported in scientific literature (PMID 22396653)

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:500. Use reported in scientific literature

Simple Western

1:10

Western Blot

1:500
Application Notes
By Western blot a band is seen at ~140 kDa. Another band is seen >200 kDa and a faint band may be seen ~80 kDa. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus of mouse brain. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 4 using NB100-2214 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Jumonji/JARID2

JARID2 (also known as Jumonji) is a nuclear protein that participates in the negative regulation of cell growth. It is required for neural tube formation and is essential for normal heart development and function. As well, it is a part of a group of proteins characterized by a novel structural motif, the JmjC domain, which is implicated in chromatin regulation through histone demethylation.

Alternate Names

JARID2, JMJ

Entrez Gene IDs

3720 (Human); 16468 (Mouse)

Gene Symbol

JARID2

UniProt

Q92833 (Human)

Additional Jumonji/JARID2 Products

Product Documents for Jumonji/JARID2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Jumonji/JARID2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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