Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free

Concentration

1.0 mg/ml

View all Jumonji/JARID2 Antibodies »

Product Summary for Jumonji/JARID2 Antibody - BSA Free

Immunogen

Partial recombinant protein made to the N-terminal portion of the human Jumanji protein (amino acids 1-159) [UniProt Q92833]

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Jumonji/JARID2 Antibody - BSA Free

Western Blot: Jumonji/JARID2 AntibodyBSA Free [NBP3-00588]

Western Blot: Jumonji/JARID2 AntibodyBSA Free [NBP3-00588]

Western Blot: Jumonji/JARID2 Antibody [NBP3-00588] - Total protein from human U2OS and NTERA-2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Jumonji (NBP3-00588) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).
Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody - BSA Free [NBP3-00588]

Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody - BSA Free [NBP3-00588]

Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NBP3-00588] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Jumonji/JARID2 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody - BSA Free [NBP3-00588]

Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody - BSA Free [NBP3-00588]

Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NBP3-00588] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Jumonji / JARID2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Jumonji / JARID2 antibody generated nuclear staining.

Applications for Jumonji/JARID2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Jumonji/JARID2

JARID2 (also known as Jumonji) is a nuclear protein that participates in the negative regulation of cell growth. It is required for neural tube formation and is essential for normal heart development and function. As well, it is a part of a group of proteins characterized by a novel structural motif, the JmjC domain, which is implicated in chromatin regulation through histone demethylation.

Alternate Names

JARID2, JMJ

Gene Symbol

JARID2

Additional Jumonji/JARID2 Products

Product Documents for Jumonji/JARID2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Jumonji/JARID2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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