Kaiso Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87866

Novus Biologicals, part of Bio-Techne
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NBP1-87866
NBP1-87866-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Kaiso Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IAELGVPLSQVKSISGTAQDGNTEPLPPDSGDKNLVIQKSKDEAQDNGATIMPIITESFSLSAEDYEMKKIIVTDSDDDDDDVIFCSEILPTKETLPSNNTVAQVQSNPGPVAISD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kaiso Antibody

Western Blot: Kaiso Antibody [NBP1-87866]

Western Blot: Kaiso Antibody [NBP1-87866]

Western Blot: Kaiso Antibody [NBP1-87866] - Analysis in human cell line RT-4, human cell line EFO-21, human cell line A-431, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: Kaiso Antibody [NBP1-87866]

Immunocytochemistry/ Immunofluorescence: Kaiso Antibody [NBP1-87866]

Immunocytochemistry/Immunofluorescence: Kaiso Antibody [NBP1-87866] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Kaiso Antibody [NBP1-87866]

Immunohistochemistry-Paraffin: Kaiso Antibody [NBP1-87866]

Immunohistochemistry-Paraffin: Kaiso Antibody [NBP1-87866] - Staining of human gallbladder shows nuclear positivity in glandular cells.

Applications for Kaiso Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF reported in scientific literature (PMID: 27765636). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kaiso

KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA (Filion et al., 2006 (PubMed 16354688)).(supplied by OMIM)

Alternate Names

KAISOkaiso transcription factor, transcriptional regulator Kaiso, WUGSC:H_DJ525N14.1, zinc finger and BTB domain containing 33, Zinc finger and BTB domain-containing protein 33, ZNF348kaiso, ZNF-kaiso

Gene Symbol

ZBTB33

Additional Kaiso Products

Product Documents for Kaiso Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Kaiso Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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