Kallikrein 3/PSA Antibody (A67-B/E3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32992
![Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (A67-B/E3) [NBP2-32992]](https://resources.bio-techne.com/images/products/Kallikrein-3-PSA-Antibody-A67-B-E3-Immunohistochemistry-Paraffin-NBP2-32992-img0001.jpg "Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (A67-B/E3) [NBP2-32992]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # A67-B/E3
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Kallikrein 3/PSA (p30) from human sperm plasma (Uniprot: P07288)
Localization
Cytoplasmic
Specificity
Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This monoclonal antibody is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This monoclonal antibody makes an excellent pair with monoclonal antibody 1A7G6B6 for PSA tests.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34549)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Kallikrein 3/PSA Antibody (A67-B/E3)
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (A67-B/E3) [NBP2-32992]
Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody (A67-B/E3) [NBP2-32992] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with PSA MAb (A67-B/E3).Applications for Kallikrein 3/PSA Antibody (A67-B/E3)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID: 33364756).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Kallikrein 3/PSA
Additional Kallikrein 3/PSA Products
Product Documents for Kallikrein 3/PSA Antibody (A67-B/E3)
Product Specific Notices for Kallikrein 3/PSA Antibody (A67-B/E3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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