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Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44048

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL9422

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P07288, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free

Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody [NBP3-44048]

Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody [NBP3-44048]

Analysis in human prostate and placenta tissues using NBP3-44048 antibody. Corresponding Kallikrein 3/PSA RNA-seq data are presented for the same tissues.
Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody [NBP3-44048]

Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody [NBP3-44048]

Staining of human placenta shows no positivity in trophoblastic cells as expected.
Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody [NBP3-44048]

Immunohistochemistry-Paraffin: Kallikrein 3/PSA Antibody [NBP3-44048]

Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Applications for Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kallikrein 3/PSA

PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liverderived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Alternate Names

KLK3, PSA

Gene Symbol

KLK3

Additional Kallikrein 3/PSA Products

Product Documents for Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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