KAP1 Antibody (4E6)
Novus Biologicals, part of Bio-Techne | Catalog # H00010155-M01
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 4E6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIER
Specificity
TRIM28 (4E6)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for KAP1 Antibody (4E6)
Western Blot: KAP1 Antibody (4E6) [H00010155-M01]
Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6. Analysis of TRIM28 expression in NIH/3T3.Immunocytochemistry/ Immunofluorescence: KAP1 Antibody (4E6) [H00010155-M01]
Immunocytochemistry/Immunofluorescence: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on HeLa cell. Antibody concentration 10 ug/ml.Immunohistochemistry-Paraffin: KAP1 Antibody (4E6) [H00010155-M01]
Immunohistochemistry-Paraffin: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.Applications for KAP1 Antibody (4E6)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation, and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: KAP1
Long Name
KRAB-associated Protein 1
Alternate Names
KRIP-1, RNF96, TF1B, TIF1B, TRIM28
Entrez Gene IDs
10155 (Human)
Gene Symbol
TRIM28
OMIM
601742 (Human)
UniProt
Additional KAP1 Products
Product Documents for KAP1 Antibody (4E6)
Product Specific Notices for KAP1 Antibody (4E6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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