KAP1 [p Ser824] Antibody (BL-246-7B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76398

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-76398
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-246-7B5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all KAP1 Antibodies »

Product Summary for KAP1 [p Ser824] Antibody (BL-246-7B5)

Immunogen

Immunogen was a synthetic phosphorylated peptide, which represented a portion of human KRAB-Associated Protein 1 surrounding Serine 824 according to the numbering given in entry NP_005753.1 (GeneID 10155)

Modification

p Ser824

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAP1 [p Ser824] Antibody (BL-246-7B5)

KAP1 [p Ser824] Antibody (BL-246-7B5)

Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] -

Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.
Western Blot: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by WB of HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Lower panel shows WB for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP1 (S824) in FFPE breast carcinoma by IHC and in FFPE EPE treated HeLa cells by ICC. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
View 4 more images

Applications for KAP1 [p Ser824] Antibody (BL-246-7B5)

Application
Recommended Usage

Flow Cytometry

Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/mg lysate

Simple Western

1:10 - 1:250

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: KAP1

KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Gene Symbol

TRIM28

Additional KAP1 Products

Product Documents for KAP1 [p Ser824] Antibody (BL-246-7B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KAP1 [p Ser824] Antibody (BL-246-7B5)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.