KAP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32071
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen this antibody was made to, maps to a region between residue 175 to 225 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_035718.2 (GeneID 21849).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for KAP1 Antibody
Western Blot: KAP1 Antibody [NBP2-32071]
Western Blot: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: KAP1 Antibody [NBP2-32071]
Immunoprecipitation: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 antibody NBP2-32071. For blotting immunoprecipitated KAP-1, NBP2-32071 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for KAP1 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KAP1
Long Name
KRAB-associated Protein 1
Alternate Names
KRIP-1, RNF96, TF1B, TIF1B, TRIM28
Gene Symbol
TRIM28
UniProt
Additional KAP1 Products
Product Documents for KAP1 Antibody
Product Specific Notices for KAP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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