Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all KAP1 Antibodies »

Product Summary for KAP1 Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 175 to 225 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_035718.2 (GeneID 21849).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAP1 Antibody

Western Blot: KAP1 Antibody [NBP2-32071]

Western Blot: KAP1 Antibody [NBP2-32071]

Western Blot: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-), NIH3T3, and Renca cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: KAP1 Antibody [NBP2-32071]

Immunoprecipitation: KAP1 Antibody [NBP2-32071]

Immunoprecipitation: KAP1 Antibody [NBP2-32071] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from TCMK-1 cells treated with 100 uM EPE for 2 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32071 used for IP at 6 ug/mg lysate. KAP-1 was also immunoprecipitated by rabbit anti-KAP-1 antibody NBP2-32071. For blotting immunoprecipitated KAP-1, NBP2-32071 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for KAP1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KAP1

KAP1 is encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Gene Symbol

TRIM28

UniProt

NP_035718.2 (Human)

Additional KAP1 Products

Product Documents for KAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.