Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all KAP1 Antibodies »

Product Summary for KAP1 [p Ser824] Antibody

Immunogen

The immunogen this antibody was made to, maps to a region of mouse KAP-1 (tripartite motif-containing 28 ) surrounding serine 824 using the numbering given in entry NP_035718.2 (Gene ID 21849) when the serine residue is phosphorylated.

Modification

p Ser824

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAP1 [p Ser824] Antibody

Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073]

Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073]

Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NBP2-32073 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using a different affinity purified rabbit anti-KAP-1 antibody at 0.4 ug/ml with an exposure time of 3 seconds.
Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073]

Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073]

Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK-1 cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32073 used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), NBP2-32073 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP-1 antibody A304-145A.

Applications for KAP1 [p Ser824] Antibody

Application
Recommended Usage

Western Blot

1:500 - 1:2500
Application Notes
Immunoprecipitation Not recommended
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KAP1

KAP1 is encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Gene Symbol

TRIM28

UniProt

NP_035718.2 (Human)

Additional KAP1 Products

Product Documents for KAP1 [p Ser824] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KAP1 [p Ser824] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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