Kappa Light Chain Antibody (5E3S7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15878

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15878-100ul
NBP3-15878-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5E3S7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Kappa Light Chain Antibodies »

Product Summary for Kappa Light Chain Antibody (5E3S7)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-107 of human Ig Kappa Chain (P01834). RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Kappa Light Chain Antibody (5E3S7)

Western Blot: Kappa Light Chain Antibody (5E3S7) [NBP3-15878]

Western Blot: Kappa Light Chain Antibody (5E3S7) [NBP3-15878]

Western Blot: Kappa Light Chain Antibody (5E3S7) [NBP3-15878] - Western blot analysis of extracts of various cell lines, using Kappa Light Chain antibody (NBP3-15878) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 1s.

Applications for Kappa Light Chain Antibody (5E3S7)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents for Kappa Light Chain Antibody (5E3S7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Kappa Light Chain Antibody (5E3S7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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