Kappa Light Chain Antibody (8O7Q9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15596

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15596-20ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8O7Q9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Kappa Light Chain Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human Kappa light chain (P01601). MDMRVLAQLLGLLLLCFPGARCDIQMTQSPSSLSASVGDRVTITCRASQGISSWLAWYQQKPEKAPKSLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYNSYP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kappa Light Chain Antibody (8O7Q9)

Western Blot: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]

Western Blot: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]

Western Blot: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596] - Western blot analysis of extracts of Human plasma cells, using Kappa Light Chain antibody (NBP3-15596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]

Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596]

Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (8O7Q9) [NBP3-15596] - Immunohistochemistry of paraffin-embedded human tonsil using Kappa Light Chain Rabbit mAb (NBP3-15596) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for Kappa Light Chain Antibody (8O7Q9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents for Kappa Light Chain Antibody (8O7Q9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Kappa Light Chain Antibody (8O7Q9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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