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Kappa Light Chain Antibody (MEM-09) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-331

Novus Biologicals, part of Bio-Techne
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NB500-331

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-09

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Crude thymus membrane fraction.

Specificity

The antibody MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with kappa light chains (22.5 kDa). Material immunoprecipitated from human serum with the antibody MEM-09 consists of IgG and traces of IgM.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kappa Light Chain Antibody (MEM-09) - BSA Free

Western Blot: Kappa Light Chain Antibody (MEM-09)BSA Free [NB500-331]

Western Blot: Kappa Light Chain Antibody (MEM-09)BSA Free [NB500-331]

Western Blot: Kappa Light Chain Antibody (MEM-09) [NB500-331] - Analysis (reducing conditions) of human blood sera using anti-human Immunoglobulin kappa-chain (MEM-09). Lane 1-3: Human blood serum of different healthy donors

Applications for Kappa Light Chain Antibody (MEM-09) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Kappa Light Chain

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Alternate Names

HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Entrez Gene IDs

3514 (Human)

Gene Symbol

IGKC

Additional Kappa Light Chain Products

Product Documents for Kappa Light Chain Antibody (MEM-09) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Kappa Light Chain Antibody (MEM-09) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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