Kappa Light Chain Antibody (RM126) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62020B
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Biotin
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM126
Concentration
1 mg/ml
Product Summary for Kappa Light Chain Antibody (RM126) [Biotin]
Immunogen
Human IgG
Specificity
This antibody reacts to the kappa light chain of human immunoglobulins, and does not react to the lambda light chain. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Kappa Light Chain Antibody (RM126) [Biotin]
Immunohistochemistry: Kappa Light Chain Antibody (RM126) [Biotin] [NBP2-62020B] - Immunohistochemistry of Human Tonsil using Anti-Kappa Light Chain antibody NBP2-62020B.
ELISA: Kappa Light Chain Antibody (RM126) [Biotin] [NBP2-62020B] - ELISA of human immunoglobulins shows NBP2-62020B reacts to the kappa light chain of human immunoglobulins. No cross reactivity with the lambda light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins.NBP2-62020B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA: Kappa Light Chain Antibody (RM126) [Biotin] [NBP2-62020B] - A titer ELISA using NBP2-62020B. The plate was coated with different amounts of human IgG4k. A serial dilution of NBP2-62020B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Applications for Kappa Light Chain Antibody (RM126) [Biotin]
Application
Recommended Usage
ELISA
0.05ug/ml - 0.2ug/ml
Flow Cytometry
0.5ug/ml-2ug/ml
Immunocytochemistry/ Immunofluorescence
0.5ug/ml - 2ug/ml
Immunohistochemistry
0.5ug/ml - 2ug/ml
Application Notes
This primary antibody can also be used as a secondary antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Kappa Light Chain
Alternate Names
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene Symbol
IGKC
Additional Kappa Light Chain Products
Product Documents for Kappa Light Chain Antibody (RM126) [Biotin]
Product Specific Notices for Kappa Light Chain Antibody (RM126) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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