Kappa Light Chain Antibody (SPM558) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45082
![Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (SPM558) - IHC-Prediluted [NBP2-45082]](https://resources.bio-techne.com/images/products/Kappa-Light-Chain-Antibody-SPM558-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-45082-img0001.jpg "Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (SPM558) - IHC-Prediluted [NBP2-45082]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM558
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Kappa Light Chain Antibody (SPM558) - IHC-Prediluted
Immunogen
Recombinant human Kappa Light Chain
Localization
Cell Surface, cytoplasmic and secreted
Specificity
This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Kappa Light Chain Antibody (SPM558) - IHC-Prediluted
Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (SPM558) - IHC-Prediluted [NBP2-45082]
Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (SPM558) - IHC-Prediluted [NBP2-45082] - Formalin-paraffin human tonsil stained with Kappa Light Chain Ab (SPM558). Note cell membrane & cytoplasmic staining.Applications for Kappa Light Chain Antibody (SPM558) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Kappa Light Chain
Alternate Names
HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene Symbol
IGKC
Additional Kappa Light Chain Products
Product Documents for Kappa Light Chain Antibody (SPM558) - IHC-Prediluted
Product Specific Notices for Kappa Light Chain Antibody (SPM558) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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