Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all p300 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:33106900).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KAT3B/p300 Antibody

Western Blot: KAT3B/p300 Antibody [NB500-161]

Western Blot: KAT3B/p300 Antibody [NB500-161]

Western Blot: KAT3B/p300 Antibody [NB500-161] - Effect of PRDX2 and PRDX4 on HIF-1alpha-p300 interaction. HeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, and exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1alpha, V5, and p300. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/abstract/7142), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161]

Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161]

Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NB500-161] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Western Blot: KAT3B/p300 Antibody [NB500-161]

Western Blot: KAT3B/p300 Antibody [NB500-161]

Western Blot: KAT3B/p300 Antibody [NB500-161] - Analysis of HeLa lysates using NB500-161. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).
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Applications for KAT3B/p300 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry

1:500 to 1:2000

Immunohistochemistry-Paraffin

1:500 to 1:2000

Immunoprecipitation

2-10 ug/mg of lysate

Western Blot

1:1000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. KAT3B/p300 antibody validated for chip from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB500-161 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: p300

p300 is highly homologous to CREB-binding protein (CBP). Like CBP, it is a conserved transcriptional coactivator and histone acetyltransferase (HAT) that links chromatin remodeling with transcription. It also plays a role in cell cycle progression.

Long Name

E1A-binding Protein p300

Alternate Names

EP300

Entrez Gene IDs

2033 (Human)

Gene Symbol

EP300

UniProt

Q09472

Additional p300 Products

Product Documents for KAT3B/p300 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KAT3B/p300 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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