KCC2/SLC12A5 [p Ser940] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29513

Novus Biologicals, part of Bio-Techne
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NBP2-29513
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all KCC2/SLC12A5 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 conjugated to KLH. Accession # Q63633

Reactivity Notes

Use in Human reported in scientific literature (PMID:34500023). Mouse reactivity reported in scientific literature (PMID: 30106375).

Modification

p Ser940

Specificity

Specific for endogenous levels of the ~135 kDa KCC2/SLC12A5 protein phosphorylated at Ser940. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KCC2/SLC12A5 [p Ser940] Antibody

Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513]

Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513]

Western Blot: KCC2/SLC12A5 [p Ser940] Antibody [NBP2-29513] - Rat hippocampal homogenate showing specific labeling of the ~ 135k KCC2 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Applications for KCC2/SLC12A5 [p Ser940] Antibody

Application
Recommended Usage

Immunoprecipitation

40 uL of antibody per 1 mg lysate

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KCC2/SLC12A5

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine. Serine 940 on KCC2 has been shown to be phosphorylated by PKC and has further been demonstrated to be the major site for PKC-dependent phosphorylation for full length KCC2 molecules when expressed in HEK-293 cells as phosphorylation of Ser940 increased the cell surface stability of KCC2 in this system by decreasing it's rate of internalization from the plasma membrane (Lee et al., 2007).

Long Name

K-Cl Cotransporter 2

Alternate Names

SLC12A5

Entrez Gene IDs

171373 (Rat)

Gene Symbol

SLC12A5

UniProt

Q63633 (Rat)

Additional KCC2/SLC12A5 Products

Product Documents for KCC2/SLC12A5 [p Ser940] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KCC2/SLC12A5 [p Ser940] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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