KCC2/SLC12A5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13161
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse KCC2/SLC12A5. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
126 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for KCC2/SLC12A5 Antibody
Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161]
Western Blot: KCC2/SLC12A5 Antibody [NBP3-13161] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: KCC2/SLC12A5 Antibody [NBP3-13161]
Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP3-13161]
Immunohistochemistry-Paraffin: KCC2/SLC12A5 Antibody [NBP3-13161] - KCC2/SLC12A5 antibody detects KCC2/SLC12A5 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: KCC2/SLC12A5 protein stained by KCC2/SLC12A5 antibody (NBP3-13161) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.Applications for KCC2/SLC12A5 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: KCC2/SLC12A5
Long Name
K-Cl Cotransporter 2
Alternate Names
SLC12A5
Gene Symbol
SLC12A5
Additional KCC2/SLC12A5 Products
Product Specific Notices for KCC2/SLC12A5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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