KCNJ15 Antibody (1B2)
Novus Biologicals, part of Bio-Techne | Catalog # H00003772-M01
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1B2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
KCNJ15 (NP_002234, 290 a.a. ~ 355 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TSAVCQSRTSYIPEEIYWGFEFVPVVSLSKNGKYVADFSQFEQIRKSPDCTFYCADSEKQQLEEKY
Specificity
KCNJ15 (1B2)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for KCNJ15 Antibody (1B2)
![Western Blot: KCNJ15 Antibody (1B2) [H00003772-M01]](https://resources.bio-techne.com/images/products/KCNJ15-Antibody-1B2-Western-Blot-H00003772-M01-img0005.jpg "Western Blot: KCNJ15 Antibody (1B2) [H00003772-M01]")
Western Blot: KCNJ15 Antibody (1B2) [H00003772-M01]
Western Blot: KCNJ15 Antibody (1B2) [H00003772-M01] - Analysis of KCNJ15 expression in transfected 293T cell line by KCNJ15 monoclonal antibody (M01), clone 1B2.Lane 1: KCNJ15 transfected lysate(42.6 KDa).Lane 2: Non-transfected lysate.![Western Blot: KCNJ15 Antibody (1B2) [H00003772-M01]](https://resources.bio-techne.com/images/products/KCNJ15-Antibody-1B2-Western-Blot-H00003772-M01-img0003.jpg "Western Blot: KCNJ15 Antibody (1B2) [H00003772-M01]")
Western Blot: KCNJ15 Antibody (1B2) [H00003772-M01]
Western Blot: KCNJ15 Antibody (1B2) [H00003772-M01] - Analysis of KCNJ15 over-expressed 293 cell line, cotransfected with KCNJ15 Validated Chimera RNAi ( Cat # H00003772-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KCNJ15 monoclonal antibody (M01), clone 1B2 (Cat # H00003772-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: KCNJ15 Antibody (1B2) [H00003772-M01] - Detection limit for recombinant GST tagged KCNJ15 is 3 ng/ml as a capture antibody.
Applications for KCNJ15 Antibody (1B2)
Application
Recommended Usage
Western Blot
1:500RNAi Validation
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: KCNJ15
Alternate Names
inward rectifier K+ channel KIR4.2, inwardly rectifing potassium channel Kir4.210ATP-sensitive inward rectifier potassium channel 15, inwardly rectifying subfamily J member 15, KCNJ14, Kir1.3, KIR4.2, MGC13584, potassium inwardly-rectifying channel, subfamily J, member 15
Entrez Gene IDs
3772 (Human)
Gene Symbol
KCNJ15
OMIM
602106 (Human)
UniProt
Additional KCNJ15 Products
Product Documents for KCNJ15 Antibody (1B2)
Product Specific Notices for KCNJ15 Antibody (1B2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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