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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # AT4C3

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human KCTD15 (1-234aa) purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for KCTD15 Antibody (AT4C3) - BSA Free

Western Blot: KCTD15 Antibody (AT4C3)BSA Free [NBP1-49429]

Western Blot: KCTD15 Antibody (AT4C3)BSA Free [NBP1-49429]

Western Blot: KCTD15 Antibody (AT4C3) [NBP1-49429] - The cell lysates (10ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanKCTD15 antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1 : 293T cell lysateLane 2 : KCTD15 Transfected 293T cell lysate
Immunocytochemistry/ Immunofluorescence: KCTD15 Antibody (AT4C3) - BSA Free [NBP1-49429]

Immunocytochemistry/ Immunofluorescence: KCTD15 Antibody (AT4C3) - BSA Free [NBP1-49429]

Immunocytochemistry/Immunofluorescence: KCTD15 Antibody (AT4C3) [NBP1-49429] - Human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
Western Blot: KCTD15 Antibody (AT4C3)BSA Free [NBP1-49429]

Western Blot: KCTD15 Antibody (AT4C3)BSA Free [NBP1-49429]

Western Blot: KCTD15 Antibody (AT4C3) [NBP1-49429] - PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Applications for KCTD15 Antibody (AT4C3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:250

Western Blot

1:250-1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KCTD15

KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.

Alternate Names

BTB/POZ domain-containing protein KCTD15, MGC25497, MGC2628, potassium channel tetramerisation domain containing 15

Entrez Gene IDs

79047 (Human)

Gene Symbol

KCTD15

Additional KCTD15 Products

Product Documents for KCTD15 Antibody (AT4C3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KCTD15 Antibody (AT4C3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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