Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C1

Concentration

1 mg/ml

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Product Specifications

Immunogen

KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)

Specificity

Detects KDEL tagged proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for KDEL Antibody (2C1)

Western Blot: KDEL Antibody (2C1) [NBP2-61926]

Western Blot: KDEL Antibody (2C1) [NBP2-61926]

Western Blot: KDEL Antibody (2C1) [NBP2-61926] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61926). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.
Immunohistochemistry: KDEL Antibody (2C1) [NBP2-61926]

Immunohistochemistry: KDEL Antibody (2C1) [NBP2-61926]

Immunohistochemistry: KDEL Antibody (2C1) [NBP2-61926] - Immunohistochemistry analysis using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Tissue: kidney. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) KDEL Antibody (D) Composite.

Applications for KDEL Antibody (2C1)

Application
Recommended Usage

Immunohistochemistry

1:25

Western Blot

1:1000
Application Notes
A 1:1000 dilution of this antibody was sufficient for detection of KDEL in 10 ug of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KDEL

he endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.

Alternate Names

Endoplasmic reticulum resident protein 58, EP58KDEL1, ER protein 58, ERp58, KDEL (Lys-Asp-Glu-Leu) containing 1, KDEL motif-containing 1, KDEL motif-containing protein 1, MGC5302

Gene Symbol

POGLUT2

Additional KDEL Products

Product Documents for KDEL Antibody (2C1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for KDEL Antibody (2C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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