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KDM2A/FBXL11 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78305

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.2 mg/ml

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human KDM2A/FBXL11 protein (between residues 350-500) [UniProt Q9Y2K7]

Localization

Nucleus, nucleoplasm. Note: Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for KDM2A/FBXL11 Antibody - BSA Free

Western Blot: KDM2A/FBXL11 AntibodyBSA Free [NBP1-78305]

Western Blot: KDM2A/FBXL11 AntibodyBSA Free [NBP1-78305]

Western Blot: KDM2A/FBXL11 Antibody [NBP1-78305] - 1) 293T. 2) 293T transiently transfected to express human KDM2A. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305]

Immunocytochemistry/ Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305]

Immunocytochemistry/Immunofluorescence: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with KCM2A/FBXL11 Antibody (NBP1-78305) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305]

Immunohistochemistry: KDM2A/FBXL11 Antibody - BSA Free [NBP1-78305]

Immunohistochemistry: KDM2A/FBXL11 Antibody [NBP1-78305] - IHC analysis of KDM2A / FBXL11 in human kidney cancer xenograft using DAB with hematoxylin counterstain.

Applications for KDM2A/FBXL11 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunohistochemistry

1:300

Immunohistochemistry-Paraffin

1:300

Western Blot

reported by customer review
Application Notes
This KDM2A/FBXL11 antibody is useful for Immunocytochemistry/Immunofluorescence where nuclear staining is observed in HeLa cells and IHC-paraffin embedded sections where nuclear staining is seen in human kidney cancer xenograft. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 3 reviews rated 4.7 using NBP1-78305 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KDM2A/FBXL11

KDM2A / FBXL11 (also known as JHDM1A, lysine specific demethylase 1A) is a F-box family member and JmjC-domain containing histone demethylase that specifically demethylates Lysine 36 of H3 (H3 K36). KDM2A / FBXL11 is involved in epigenetic silencing and required to maintain the heterochromatic state, as well as sustaining centromeric integrity and genomic stability during mitosis (PMID 19001877). Under conditions of starvation, FBXL11's ability to repress RNA transcription has been shown to be reduced (PMID 20379134). In addition, KDM2A / FBXL11 is also a key inhibitory regulator of NF kappa B activity. This regulation occurs when K218 and K221 of the p65 subunit are methylated. The overexpression of KDM2A / FBXL11 can decrease the viability of specific cancer cell lines, while downregulation was shown to have the opposite effect (PMID 20080798). KDM2A / FBXL11 shows further regulatory involvement at CpG islands. Nonmethylated CpG islands preferentially bind KDM2A / FBXL11 using a zinc finger domain, in which the binding is blocked when CpG DNA is in its methlylated form (PMID 20417597).

Long Name

Lysine-specific demethylase 2A

Alternate Names

CXXC8, EC 1.14.11.27, F-box protein FBL7, FBL11, FBL7, FBXL11, JHDM1A, KDM2A, KIAA1004

Entrez Gene IDs

22992 (Human)

Gene Symbol

KDM2A

UniProt

Additional KDM2A/FBXL11 Products

Product Documents for KDM2A/FBXL11 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for KDM2A/FBXL11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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