Keap1 Antibody (OTI1B4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71496

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Keap1 Antibodies »

Product Summary for Keap1 Antibody (OTI1B4) - Azide and BSA Free

Immunogen

Full length human recombinnt protein of human KEAP1(NP_987096) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

69.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Keap1 Antibody (OTI1B4) - Azide and BSA Free

Western Blot: Keap1 Antibody (OTI1B4)Azide and BSA Free [NBP2-71496]

Western Blot: Keap1 Antibody (OTI1B4)Azide and BSA Free [NBP2-71496]

Western Blot: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).
Immunocytochemistry/ Immunofluorescence: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496]

Immunocytochemistry/ Immunofluorescence: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496]

Immunocytochemistry/Immunofluorescence: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Staining of 293T cells transfected by pCMV6-ENTRY KEAP1 using anti-KEAP1 antibody (green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).
Immunohistochemistry: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496]

Immunohistochemistry: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496]

Immunohistochemistry: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Staining of paraffin-embedded Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.
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Applications for Keap1 Antibody (OTI1B4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Keap1

The KEAP1 gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associatedprotein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins inthe cytoplasm is followed

Long Name

Kelch-like ECH-associated Protein 1

Alternate Names

INrf2

Gene Symbol

KEAP1

Additional Keap1 Products

Product Documents for Keap1 Antibody (OTI1B4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Keap1 Antibody (OTI1B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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