Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rabbit

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8D5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Ki67/MKI67 Antibodies »

Product Summary for Ki67/MKI67 Antibody (8D5) - Azide and BSA Free

Immunogen

The immunogen for this KI67/MKI67 Antibody (8D5) was made using a synthetic peptide from the internal region of Human KI67/MKI67 (amino acids: 1100-1113, 1222-1235 and 3 more spots); sequence: CEDLAGFKELFQTPG .

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

359 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ki67/MKI67 Antibody (8D5) - Azide and BSA Free

Western Blot: Ki67/MKI67 Antibody (8D5)Azide and BSA Free [NBP2-80821]

Western Blot: Ki67/MKI67 Antibody (8D5)Azide and BSA Free [NBP2-80821]

Western Blot: Ki67/MKI67 Antibody (8D5) - Azide and BSA Free [NBP2-80821] - Analysis using Ki67 mouse mAb against Hela (1), MCF-7 (2) and Raji (3) cell lysate. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (8D5) - Azide and BSA Free [NBP2-80821]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (8D5) - Azide and BSA Free [NBP2-80821]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (8D5) - Azide and BSA Free [NBP2-80821] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Ki67/MKI67 Antibody (8D5) NBP2-80821 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (8D5) - Azide and BSA Free [NBP2-80821]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (8D5) - Azide and BSA Free [NBP2-80821]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (8D5) - Azide and BSA Free [NBP2-80821] - Analysis of paraffin-embedded lung cancer (left) and rectal cancer (right) using Ki67 mouse mAb with DAB staining. Image from the standard format of this antibody.
View 4 more images

Applications for Ki67/MKI67 Antibody (8D5) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:100. Use reported by customer review

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ki67/MKI67

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126

Gene Symbol

MKI67

Additional Ki67/MKI67 Products

Product Documents for Ki67/MKI67 Antibody (8D5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ki67/MKI67 Antibody (8D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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