Ki67/MKI67 Antibody (CL1234)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76476

Novus Biologicals, part of Bio-Techne
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NBP2-76476
NBP2-76476-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1234

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ki67/MKI67 Antibodies »

Product Specifications

Immunogen

This KI67/MKI67 Antibody was developed against Recombinant Protein corresponding to amino acids 1-159 from Human KI67/MKI67: DGPHFPLSLSTCLFGRGIECDIRIQLPVVSKQHCKIEIHEQEAILHNFSSTNPTQVNGSVIDEPVRLKHGDVITIIDRSFRYENESLQSGRKSTEFPRKIREQEPARRVSRSSFSSDPDEKAQDSKAYSKITEGKVSGNPQVHI

Epitope

PLSLSTCLFGRGIEC

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

359 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ki67/MKI67 Antibody (CL1234)

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (CL1234) [NBP2-76476]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (CL1234) [NBP2-76476]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (CL1234) [NBP2-76476] - Staining of U-251 cells using the Anti-MK167 monoclonal antibody, showing specific staining in the nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (CL1234) [NBP2-76476]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (CL1234) [NBP2-76476]

Immunohistochemistry-Paraffin: Ki67/MKI67 Antibody (CL1234) [NBP2-76476] - Staining of human uterus shows nuclear immunoreactivity in a subset of glanular cells.
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (CL1234) [NBP2-76476]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody (CL1234) [NBP2-76476]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody (CL1234) [NBP2-76476] - Staining of A-431 cells using the Anti-MK167 monoclonal antibody, showing specific staining in the nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
View 4 more images

Applications for Ki67/MKI67 Antibody (CL1234)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ki67/MKI67

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126

Gene Symbol

MKI67

Additional Ki67/MKI67 Products

Product Documents for Ki67/MKI67 Antibody (CL1234)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ki67/MKI67 Antibody (CL1234)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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