Ki67/MKI67 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05538

Novus Biologicals, part of Bio-Techne
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NBP3-05538-50ul
NBP3-05538-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ki67/MKI67 Antibodies »

Product Specifications

Immunogen

Mixture of recombinant human Ki67/MKI67 constructs expressing the N-terminal region, amino acids 1-300 and an internal region, amino acids 1111-1490

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Scientific Data Images for Ki67/MKI67 Antibody

Western Blot: Ki67/MKI67 Antibody [NBP3-05538]

Western Blot: Ki67/MKI67 Antibody [NBP3-05538]

Western Blot: Ki67/MKI67 Antibody [NBP3-05538] - Western blot analysis of equal amounts of cell lysates probed with Ki67/MKI67 Antibody at a dilution of 1:5,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67/MKI67 isoforms of molecular weight 345kDa and 395kDa. Since Ki67/MKI67 is a rather unstable snd sjhort half life protein multiple proteolytic fragments of the two isoforms are also detected on the blot.
Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody [NBP3-05538]

Immunocytochemistry/ Immunofluorescence: Ki67/MKI67 Antibody [NBP3-05538]

Immunocytochemistry/Immunofluorescence: Ki67/MKI67 Antibody [NBP3-05538] - Immunofluorescent analysis of HeLa cells stained with Ki67/MKI67 Antibody at a dilution of 1:2,000 in red, and mouse mAb to beta-tubulin at a dilution of 1:5,000 in green. The blue is DAPI staining of nuclear DNA. The Ki67/MKI67 antibody detects Ki67/MKI67 protein predominantly expressed in nucleoli of cells in interphase and in mitotic cells Ki67/MKI67 forms a coat around condensed chromosomes. Ki67/MKI67 is not detected in cells in the quiescent G0 state. The beta-tubulin antibody produces strong staining of cytoplasmic microtubules.

Applications for Ki67/MKI67 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000-5000

Western Blot

1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgY purified

Formulation

Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.

Preservative

0.035% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Ki67/MKI67

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Long Name

Antigen Identified by Monoclonal Antibody Ki67

Alternate Names

Ki-67, KIA, MIB-1, MKI67, PPP1R105, TSG126

Gene Symbol

MKI67

Additional Ki67/MKI67 Products

Product Documents for Ki67/MKI67 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ki67/MKI67 Antibody

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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